![]() ![]() HIV-1 antibody testing strategy: evaluation of ELISA screening and western blot profiles in a mixed low risk/high risk patient population. HTLV-II sequences were detected by PCR in one of the Western blot-positive samples, as well as in one Western blot-indeterminate sample that showed reactivity to p24 only. 10.1086/341985ĭownie J, Howard R, Bowcock B, Cunningham A. Of the 61 repeatably reactive samples, 2 were positive, 26 were negative, and 33 were interpreted as indeterminate on Western blot. Discrepant results of 2 screening tests for anti-HIV antibody. Stickle DF, Pirruccello SJ, Swindells S, Hinrichs SH. Journal of the International AIDS Society 2011 14(1):18. Indeterminate and discrepant rapid HIV test results in couples' HIV testing and counselling centres in Africa. Indian journal of medical microbiology 2008 26(3):284–285. Comparison of ELISA and rapid screening tests for the diagnosis of HIV, hepatitis B and hepatitis C among healthy blood donors in a tertiary care hospital in Mumbai. Comparison of ELISA and rapid screening tests for the diagnosis of HIV in high risk individuals. This algorithm produces fewer indeterminate results and has a faster turnaround time for HIV-1 antibody positive samples compared to previous algorithms that utilized the Western blot (WB) for confirmation. Most indeterminate results became HIV-negative over time, but follow-up is still needed to detect positive serologies.Īfolabi AF, Fasanu AO, Adeyemi AS. HIV surveillance programs should develop a protocol for reporting individuals with mixed or persistently indeterminate HIV results on multiple follow-up visits. HIV serologically indeterminate results were associated with gender and marital status. The proportions of persons with indeterminate results progressing to HIV positive, negative or indeterminate results in subsequent visits was 5%, 71% and 24%, respectively. The frequency of indeterminate results for both HSV-1 and HSV-2 may be rising possibly due to increased testing in the setting of declining seroprevalence. Indeterminate results were less likely among women than men (adjPR 0.76, 95% CI 0.71,0.81), in unmarried participants than married participants (adjPR 0.92, 95% CI 0.85,99), and in individuals with primary (adjPR 0.90, 95% CI 0.80,1.02), secondary (adjPR 0.83, 95% CI 0.73,0.96) and post-secondary (adjPR 0.75, 95% CI 0.60,0.94) education, relative to no education. The prevalence of HIV serologically indeterminate results was 4.9%. Modified Poisson regression models with generalized estimating equations were used to assess prevalence ratios (PRs) of subsequent HIV serological outcomes and factors associated with HIV indeterminate results. Indeterminate results were defined as contradictory EIAs. We assessed future HIV serological outcomes for individuals with indeterminate results and associated factors in Rakai, Uganda.ġ15,944 serological results, defined by two enzyme immunoassay (EIAs), among 39,440 individuals aged 15-49 years in the Rakai Community Cohort Study were assessed. doi:10.1128/CVI.Indeterminate HIV test results are common, but little is known about the evolution of indeterminate serology and its sociodemographic and behavioral correlates. Frequency, causes, and new challenges of indeterminate results in Western blot confirmatory testing for antibodies to human immunodeficiency virus. ![]() Western blot-based logistic regression model for the identification of recent HIV-1 infection: A promising HIV-1 surveillance approach for resource-limited regions. Western Blot INDETERMINATE was always accompanied with detectable RNA of HIV-1 with cases of true infection. Laboratory Testing for the Diagnosis of HIV Infection: Updated Recommendations. In addition, this laboratory stage of having. Background: Most enzyme immunoassay-reactive specimens producing indeterminate Western blot results belong to individuals who are not infected with human immunodeficiency virus type 1 (HIV-1). doi:10.1016/j.jcv.2013.10.006Ĭenters for Disease Control and Prevention and Association of Public Health Laboratories. Applying the Western Blot (WB) technique, screening for HTLV I/II in blood donors has shown incomplete antibody reactivity against viral antigens, which has. However, indeterminate WB reactivity to HIV-1 proteins may occur in individuals who do not appear to be infected with HIV. The Multispot rapid HIV-1/HIV-2 differentiation assay is comparable with the Western blot and an immunofluorescence assay at confirming HIV infection in a prospective study in three regions of the United States. The Western blot (WB) assay is the most widely accepted confirmatory assay for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1). ![]()
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